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Mutation of Y223 to the phenylalanine residue resulted inside a mutant with just about the same catalytic action as being the wild style enzyme and so this residue is not major for that binding of substrate in NagA.
m for that E131Q mutant is elevated to three.six mM. A significant portion of the reduction in catalytic performance has arisen as the metallic material of your purified proteins is fairly small.
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Residues Q59 and N61 were being mutated together to a pair of histidine residues to build an HxH motif that is certainly analogous to that found in NagA from B. subtilis
The precise function from the metallic cofactor is of particular interest for NagA as link alternatif naga26 the two crystal buildings available for the enzyme from E. coli